Objective: The current study explored the correlation of Helicobacter pylori and the polymorphisms of human leukocyte antigen II (HLA-II) alleles with Graves disease (GD).
Methods: A total of 216 patients with GD were recruited. 102 healthy volunteers constituted the control group. Levels of H. pylori immunoglobulin G (IgG) antibodies and H. pylori cytotoxin-associated gene A (CagA) IgG antibodies were detected using enzyme-linked immunosorbent assays. Molecular typing of the HLA-II alleles was conducted using polymerase chain reaction with sequence specific primers.
Results: H. pylori, particularly CagA-positive strains, HLA-DQA1 0201, and HLA-DQA1 0501 were associated with GD (P=0.015, OR=1.811; P=0.000, OR=3.085; P=0.000, OR=0.315; and P=0.004, OR=2.844, respectively). Patients with CagA-positive H. pylori and negative HLA-DQA1 0201 or positive HLA-DQA1 0501 were more likely exposed to GD compared with those with only one of these indices.
Conclusion: CagA-positive H. pylori, negative HLA-DQA1 0201, or positive HLA-DQA1 0501 may increase the risk of GD.
Keywords: Autoimmune thyroid disease; CI; CagA; EDTA; Ethylene Diamine Tetraacetic Acid; FT3; FT4; GD; Graves disease; H. pylori; HLA; Helicobacter pylori; Human leukocyte antigen; IgG; Immunoglobulin G; OR; PCR; SPSS; Statistical Product and Service Solutions; TPOAb; TRAb; TSH; TgAb; confidence intervals; cytotoxin-associated gene A; dNTP; deoxy-ribonucleoside triphosphate; free thyroxine; free triiodothyronine; human leukocyte antigen; odds ratio; polymerase chain reaction; thyroglobulin antibody; thyroid peroxidase antibody; thyroid stimulating hormone; thyrotrophin receptor antibody.
© 2013.