Recombinant Antibodies to Histone Post-Translational Modifications

Nat Methods. 2013 Oct;10(10):992-5. doi: 10.1038/nmeth.2605. Epub 2013 Aug 18.

Abstract

Variability in the quality of antibodies to histone post-translational modifications (PTMs) is a widely recognized hindrance in epigenetics research. Here, we produced recombinant antibodies to the trimethylated lysine residues of histone H3 with high specificity and affinity and no lot-to-lot variation. These recombinant antibodies performed well in common epigenetics applications, and enabled us to identify positive and negative correlations among histone PTMs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / genetics
  • Antibodies / immunology*
  • Antibody Affinity*
  • Binding Sites, Antibody
  • Cell Line
  • Escherichia coli / genetics
  • Histones / chemistry
  • Histones / genetics
  • Histones / immunology*
  • Humans
  • Lysine / chemistry
  • Lysine / genetics
  • Lysine / immunology*
  • Peptide Library
  • Protein Processing, Post-Translational*
  • Sensitivity and Specificity
  • Single-Chain Antibodies / genetics
  • Single-Chain Antibodies / immunology

Substances

  • Antibodies
  • Histones
  • Peptide Library
  • Single-Chain Antibodies
  • Lysine