Abstract
Minor histocompatibility antigen HA-1-specific cytotoxic lymphocyte (CTL) clones have apparent anti-leukaemic efficacy, and the AML/ETO gene is a special fusion gene in leukaemic cells. Thus, we hypothesised that a vaccine targeting HA-1 and AML/ETO could stimulate NK cells to target leukaemia cells. Furthermore, we packaged the vaccine using attenuated Salmonella to enhance its immuno-activity. Expression of the NK cell-activating ligand ULBP2 was notably elevated upon packaging in a co-recombinant group. An AML/ETO single plasmid gave the weakest vaccine. The level of miR-182, which targets ULBP2, significantly decreased with increasing IFN-γ and granzyme B in a co-recombinant group. In summary, DNA vaccines including AML/ETO and HA-1 fragments significantly enhance the innate immunity of NK cells in vitro.
Keywords:
DNA vaccine; NK cells; Salmonella; innate immunity.
© 2013 International Federation for Cell Biology.
MeSH terms
-
Apoptosis
-
Bacterial Vaccines / immunology*
-
Cells, Cultured
-
GPI-Linked Proteins / genetics
-
GPI-Linked Proteins / metabolism
-
Granzymes / metabolism
-
HEK293 Cells
-
Humans
-
Immunity, Innate*
-
Intercellular Signaling Peptides and Proteins / genetics
-
Intercellular Signaling Peptides and Proteins / metabolism
-
Interferon-gamma / metabolism
-
Killer Cells, Natural / cytology
-
Killer Cells, Natural / immunology*
-
Killer Cells, Natural / metabolism
-
Leukemia, Myeloid, Acute / immunology
-
Leukemia, Myeloid, Acute / pathology
-
MicroRNAs / metabolism
-
Minor Histocompatibility Antigens / genetics
-
Minor Histocompatibility Antigens / metabolism
-
Oligopeptides / genetics
-
Oligopeptides / metabolism
-
Proto-Oncogene Proteins / genetics
-
Proto-Oncogene Proteins / metabolism
-
RNA, Messenger / metabolism
-
RUNX1 Translocation Partner 1 Protein
-
Recombinant Fusion Proteins / genetics
-
Salmonella / metabolism*
-
Transcription Factors / genetics
-
Transcription Factors / metabolism
-
Vaccines, DNA / immunology*
Substances
-
Bacterial Vaccines
-
GPI-Linked Proteins
-
HA-1 antigen
-
Intercellular Signaling Peptides and Proteins
-
MicroRNAs
-
Minor Histocompatibility Antigens
-
Mirn182 microRNA, human
-
Oligopeptides
-
Proto-Oncogene Proteins
-
RNA, Messenger
-
RUNX1 Translocation Partner 1 Protein
-
RUNX1T1 protein, human
-
Recombinant Fusion Proteins
-
Transcription Factors
-
ULBP2 protein, human
-
Vaccines, DNA
-
Interferon-gamma
-
Granzymes