Dietary grape seed extract ameliorates symptoms of inflammatory bowel disease in IL10-deficient mice

Mol Nutr Food Res. 2013 Dec;57(12):2253-7. doi: 10.1002/mnfr.201300146. Epub 2013 Aug 21.


Grape seed extract (GSE) is a by-product of the wine industry, with abundant polyphenolic compounds known for their anti-inflammatory and anti-oxidative effects. Using IL10-deficient mice (IL10KO), here we showed that GSE (1% of dry feed weight) ameliorated inflammatory bowel disease indices, increased colonic goblet cell numbers and decreased myeloperoxidase levels in the large intestine. Concomitantly, GSE supplementation attenuated inflammation, decreased the expression of pore forming tight junction protein claudin2, and increased levels of Lactobacilli and Bacteroides in the gut microbiota of IL10KO mice. In summary, our study shows that GSE has protective roles on inflammatory bowel disease through altering gut inflammation, tight junction protein expression, and gut microbiota composition.

Keywords: Epithelium; Grape seed extract; IL-10; Inflammatory bowel disease; Intestine; Microbiota.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bacteroides / drug effects
  • Colon / cytology
  • Colon / drug effects
  • Gastrointestinal Tract / drug effects
  • Gastrointestinal Tract / microbiology
  • Goblet Cells / drug effects
  • Grape Seed Extract / pharmacology*
  • Inflammatory Bowel Diseases / diet therapy*
  • Inflammatory Bowel Diseases / microbiology
  • Inflammatory Bowel Diseases / pathology
  • Interferon-gamma / genetics
  • Interleukin-10 / genetics*
  • Interleukin-10 / metabolism
  • Interleukin-6 / genetics
  • Lactobacillus / drug effects
  • Mice
  • Mice, Knockout
  • Microbiota
  • Tumor Necrosis Factor-alpha / genetics


  • Grape Seed Extract
  • IL10 protein, mouse
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Interferon-gamma