Adrenomedullin Increased the Short-Circuit Current in the Pig Oviduct Through Chloride Channels via the CGRP Receptor: Mediation by cAMP and Calcium Ions but Not by Nitric Oxide

Biol Reprod. 2013 Oct 24;89(4):99. doi: 10.1095/biolreprod.113.109934. Print 2013 Oct.


The oviduct serves as a site for the fertilization of the ovum and the transport of the conceptus down to the uterus for implantation. In this study, we investigated the presence of adrenomedullin (ADM) and its receptor component proteins in the pig oviduct. The effect of ADM on oviductal secretion, the specific receptor, and the mechanisms involved were also investigated. The presence of ADM and its receptor component proteins in the pig oviduct were confirmed using immunostaining. Short-circuit current (I(sc)) technique was employed to study chloride ion secretion in the oviductal epithelium. ADM increased I(sc) through cAMP- and calcium-activated chloride channels, and this effect could be inhibited by the CGRP receptor antagonist, hCGRP8-37. In contrast, the nitric oxide synthase inhibitor, L-NG-nitroarginine methyl ester (L-NAME), could not block the effect of ADM on I(sc). In summary, ADM may increase oviductal fluid secretion via chloride secretion independent of the nitric oxide pathway for the transport of sperm and the conceptus.

Keywords: adrenal medulla; calcium; chloride channel; cyclic adenosine monophosphate (cAMP); ion channels; nitric oxide pathway; oviduct; short-circuit current.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenomedullin / analogs & derivatives
  • Adrenomedullin / metabolism*
  • Animals
  • Calcitonin Gene-Related Peptide / pharmacology
  • Calcitonin Gene-Related Peptide Receptor Antagonists
  • Calcium Signaling* / drug effects
  • Cell Membrane / drug effects
  • Cell Membrane / metabolism*
  • Chloride Channels / antagonists & inhibitors
  • Chloride Channels / metabolism*
  • Cyclic AMP / antagonists & inhibitors
  • Cyclic AMP / metabolism*
  • Enzyme Inhibitors / pharmacology
  • Estrus
  • Female
  • Humans
  • In Vitro Techniques
  • Membrane Potentials / drug effects
  • Membrane Transport Modulators / pharmacology
  • Nitric Oxide / antagonists & inhibitors
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Nitric Oxide Synthase / metabolism
  • Oviducts / cytology
  • Oviducts / drug effects
  • Oviducts / metabolism*
  • Patch-Clamp Techniques
  • Peptide Fragments / pharmacology
  • Receptors, Adrenomedullin / antagonists & inhibitors
  • Receptors, Adrenomedullin / metabolism
  • Receptors, Calcitonin Gene-Related Peptide / metabolism*
  • Second Messenger Systems / drug effects
  • Sus scrofa


  • Calcitonin Gene-Related Peptide Receptor Antagonists
  • Chloride Channels
  • Enzyme Inhibitors
  • Membrane Transport Modulators
  • Peptide Fragments
  • Receptors, Adrenomedullin
  • Receptors, Calcitonin Gene-Related Peptide
  • calcitonin gene-related peptide (8-37)
  • Adrenomedullin
  • Nitric Oxide
  • Cyclic AMP
  • Nitric Oxide Synthase
  • Calcitonin Gene-Related Peptide