A Tet-on system for DRD1-expressing cells

PLoS One. 2013 Aug 13;8(8):e72681. doi: 10.1371/journal.pone.0072681. eCollection 2013.


Cells expressing the dopamine D1 receptor (DRD1) have significant functional roles in diverse physiological processes including locomotion and drug addiction. The present work presents a novel in vivo DRD1-Bacterial Artificial Chromosome (BAC) Tet-on system allowing for the inducible activation of tet-operated transgenes specifically within DRD1-expressing cells of transgenic mice. It is shown that the DRD1-rtTA BAC-driven expression of a tet-operated reporter is under tight regulation by doxycycline and is restricted to DRD1-expressing brain regions. The model will be a useful research tool in studies of movement and reward and associated pathologies such as Parkinson's disease and addiction.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Brain / metabolism
  • Cell Line
  • Chromosomes, Artificial, Bacterial
  • Gene Expression Regulation / drug effects*
  • Gene Order
  • Genes, Reporter
  • Genetic Vectors
  • Male
  • Mice
  • Mice, Transgenic
  • Promoter Regions, Genetic
  • Receptors, Dopamine D1 / genetics*
  • Receptors, Dopamine D1 / metabolism
  • Tetracycline / pharmacology*
  • Transcriptional Activation / drug effects


  • Receptors, Dopamine D1
  • Tetracycline