GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling

Nature. 2013 Aug 22;500(7463):422-6. doi: 10.1038/nature12478.

Abstract

Wounded leaves communicate their damage status to one another through a poorly understood process of long-distance signalling. This stimulates the distal production of jasmonates, potent regulators of defence responses. Using non-invasive electrodes we mapped surface potential changes in Arabidopsis thaliana after wounding leaf eight and found that membrane depolarizations correlated with jasmonate signalling domains in undamaged leaves. Furthermore, current injection elicited jasmonoyl-isoleucine accumulation, resulting in a transcriptome enriched in RNAs encoding key jasmonate signalling regulators. From among 34 screened membrane protein mutant lines, mutations in several clade 3 GLUTAMATE RECEPTOR-LIKE genes (GLRs 3.2, 3.3 and 3.6) attenuated wound-induced surface potential changes. Jasmonate-response gene expression in leaves distal to wounds was reduced in a glr3.3 glr3.6 double mutant. This work provides a genetic basis for investigating mechanisms of long-distance wound signalling in plants and indicates that plant genes related to those important for synaptic activity in animals function in organ-to-organ wound signalling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arabidopsis / drug effects
  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cyclopentanes / metabolism
  • Cyclopentanes / pharmacology
  • Electric Conductivity
  • Electrophysiological Phenomena
  • Gene Expression Regulation, Plant / drug effects
  • Genes, Plant*
  • Herbivory / physiology
  • Isoleucine / analogs & derivatives
  • Isoleucine / metabolism
  • Models, Animal
  • Mutation
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Oxylipins / metabolism
  • Oxylipins / pharmacology
  • Plant Diseases / etiology
  • Plant Diseases / genetics
  • Plant Growth Regulators / metabolism
  • Plant Growth Regulators / pharmacology
  • Plant Leaves / drug effects
  • Plant Leaves / genetics*
  • Plant Leaves / metabolism*
  • Receptors, Glutamate* / genetics
  • Receptors, Glutamate* / metabolism
  • Signal Transduction* / drug effects
  • Signal Transduction* / genetics
  • Synapses / metabolism
  • Synaptic Transmission
  • Transcriptome / drug effects
  • Transcriptome / genetics

Substances

  • Arabidopsis Proteins
  • Cyclopentanes
  • GLR3.3 protein, Arabidopsis
  • JAS1 protein, Arabidopsis
  • Nuclear Proteins
  • Oxylipins
  • Plant Growth Regulators
  • Receptors, Glutamate
  • jasmonoyl-isoleucine
  • Isoleucine
  • jasmonic acid

Associated data

  • GEO/GSE41779