Differences in phosphorylation of phosphoglucomutase 1 in beef steaks from the longissimus dorsi with high or low star probe values

M J Anderson; S M Lonergan; E Huff-Lonergan

doi:10.1016/j.meatsci.2013.07.017

Differences in phosphorylation of phosphoglucomutase 1 in beef steaks from the longissimus dorsi with high or low star probe values

Meat Sci. 2014 Jan;96(1):379-84. doi: 10.1016/j.meatsci.2013.07.017. Epub 2013 Jul 19.

Authors

M J Anderson¹, S M Lonergan, E Huff-Lonergan

Affiliation

¹ Meat Science and Muscle Biology Group, Department of Animal Science, Iowa State University, Ames, IA 50011, United States.

PMID: 23973564
DOI: 10.1016/j.meatsci.2013.07.017

Abstract

The objective of this study was to use proteomics to identify alterations of proteins that are related to tenderness. The longissimus dorsi (LD) were removed from ten beef carcasses at 24 h postmortem, and the two with the highest (HSP; average kg of force=6.57) and lowest star probe values (LSP; average kg of force=3.75) at 14 days postmortem were identified. Two-dimensional PAGE was used to compare the sarcoplasmic fraction of the LD from HSP and LSP steaks. A series of spots identified as phosphoglucomutase 1 (PGM1) were identified. Only the most alkaline isoform was identified as being unphosphorylated. The least phosphorylated isoform (isoform 5) had a greater density of the total protein (P<0.05) and phosphorylated protein (P<0.05) in the samples from HSP steaks compared to the samples from LSP steaks. This study illustrates the importance of identifying posttranslational modifications of proteins in the search for biomarkers.

Keywords: Beef tenderness; Phosphoglucomutase; Phosphorylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cattle
Meat / analysis*
Muscle, Skeletal / enzymology*
Phosphoglucomutase / metabolism*
Phosphorylation
Proteomics

Substances

Phosphoglucomutase