The impact of discrete compartments of a multi-compartment collagen-GAG scaffold on overall construct biophysical properties

J Mech Behav Biomed Mater. 2013 Dec;28:26-36. doi: 10.1016/j.jmbbm.2013.07.016. Epub 2013 Jul 26.


Orthopedic interfaces such as the tendon-bone junction (TBJ) present unique challenges for biomaterials development. Here we describe a multi-compartment collagen-GAG scaffold fabricated via lyophilization that contains discrete mineralized (CGCaP) and non-mineralized (CG) regions joined by a continuous interface. Modifying CGCaP preparation approaches, we demonstrated scaffold variants of increasing mineral content (40 vs. 80wt% CaP). We report the impact of fabrication parameters on microstructure, composition, elastic modulus, and permeability of the entire multi-compartment scaffold as well as discrete mineralized and non-mineralized compartments. Notably, individual mineralized and non-mineralized compartments differentially impacted the global properties of the multi-compartment composite. Of particular interest for the development of mechanically-loaded multi-compartment composites, the elastic modulus and permeability of the entire construct were governed primarily by the non-mineralized and mineralized compartments, respectively. Based on these results we hypothesize spatial variations in scaffold structural, compositional, and mechanical properties may be an important design parameter in orthopedic interface repair.

Keywords: Biophysical properties; Collagen scaffold; Mineral interface; Multi-compartment.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Biophysical Phenomena*
  • Collagen / chemistry*
  • Collagen / metabolism*
  • Glycosaminoglycans / chemistry*
  • Glycosaminoglycans / metabolism*
  • Mechanical Phenomena
  • Permeability
  • Tissue Scaffolds / chemistry*
  • X-Ray Microtomography


  • Glycosaminoglycans
  • Collagen