Expression of co-stimulatory molecule B7-H4 in patients suffering from rheumatoid arthritis

Immunol Lett. Jul-Aug 2013;154(1-2):25-30. doi: 10.1016/j.imlet.2013.07.009. Epub 2013 Aug 22.


B7-H4, an inhibitory modulator of T-cell response, is one of the most recently identified cell surface molecules in the B7-CD28 signaling pathway. However, its role in the pathogenesis of rheumatoid arthritis (RA) remains unclear. In the present study, the immunofluorescence staining, confocal laser scanning microscopy and flow cytometry techniques were used to characterize B7-H4 protein expression in RA synovium tissues and peripheral blood mononuclear cell (PBMC) subsets, respectively. Our data showed that the immunolocalization of B7-H4 could be found on the membrane and in the cytoplasm of synoviocytes and CD19(+) B cells in rheumatoid synovium tissues, while B7-H4 was weakly or negatively expressed on CD3(+) T cells, CD14(+) monocytes and CD68(+) macrophages. Moreover, B7-H4 expression was observed in CD34(+) endothelial cells of neovessels in rheumatoid synovium. Flow cytometric analysis also showed that positive B7-H4 expression was found in CD19(+) B cells and CD14(+) monocytes, but not in CD3(+) T cells. Thus, our work identified the expression pattern of B7-H4 in the synovium tissues and PBMC subsets from RA patients, suggesting that B7-H4 involves in the pathological changes of rheumatoid synovium in RA progression, and its detailed biological function needs further investigations.

Keywords: B cells; B7-H4; Immunofluorescence; Rheumatoid arthritis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / metabolism
  • Arthritis, Rheumatoid / immunology*
  • B-Lymphocytes / immunology*
  • CD18 Antigens / metabolism
  • Cell Membrane / metabolism*
  • Cell Separation
  • Cells, Cultured
  • Cytoplasm / metabolism*
  • Endothelial Cells / immunology*
  • Flow Cytometry
  • Humans
  • Immunity, Cellular
  • Immunomodulation
  • Lipopolysaccharide Receptors / metabolism
  • Monocytes / immunology*
  • Protein Transport
  • Signal Transduction
  • Synovial Membrane / immunology
  • Synovial Membrane / metabolism*
  • T-Lymphocytes / immunology
  • V-Set Domain-Containing T-Cell Activation Inhibitor 1 / genetics
  • V-Set Domain-Containing T-Cell Activation Inhibitor 1 / immunology
  • V-Set Domain-Containing T-Cell Activation Inhibitor 1 / metabolism*


  • Antigens, CD34
  • CD18 Antigens
  • Lipopolysaccharide Receptors
  • V-Set Domain-Containing T-Cell Activation Inhibitor 1
  • VTCN1 protein, human