Can vitamin E and selenium alleviate the immunologic impact of aluminium on pregnant rats' spleens?

Cell Immunol. 2013 Jul-Aug;284(1-2):104-10. doi: 10.1016/j.cellimm.2013.07.019. Epub 2013 Aug 6.


This study aimed to assess if simultaneous administration of vitamin E, selenium and aluminium could alleviate the latter's immunologic impact on the spleen. Twenty eight virgin albino Sprague Dawley rats were randomly divided into two main groups: control (n=12) and experimental (n=18). These groups were divided into two subgroups each. The first control group received distiled water and the second one vitamin E and selenium for 3months through intragastric tubes. Of the two experimental subgroups, the first received oral aluminium chloride (AlCl3) at a dose 150mg/kg of body weight/day, and the second received AlCl3 plus vitamin E and selenium for 3months. Sixteen (out of eighteen) subject rats became pregnant. At day 20 of gestation, dams were sacrificed, and spleens were dissected and processed for histologic examination with routine and immunohistologic staining and semi-quantitative assessment. Aluminium administration induced a significant decrease in the number and density of T-lymphocytes and macrophages in the spleen as well as splenic fibrosis during pregnancy. Vitamin E and selenium reduced but did not completely prevent these changes.

Keywords: Macrophages; Pregnancy; Selenium; Spleen; T-lymphocytes; Vitamin E.

MeSH terms

  • Aluminum Chloride
  • Aluminum Compounds / immunology
  • Aluminum Compounds / toxicity*
  • Animals
  • Antioxidants / pharmacology*
  • Chlorides / immunology
  • Chlorides / toxicity*
  • Female
  • Fibrosis / chemically induced
  • Immunohistochemistry
  • Pregnancy
  • Random Allocation
  • Rats
  • Rats, Sprague-Dawley
  • Selenium / pharmacology*
  • Spleen / cytology
  • Spleen / drug effects*
  • Spleen / immunology*
  • Spleen / pathology
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*
  • Vitamin E / pharmacology*


  • Aluminum Compounds
  • Antioxidants
  • Chlorides
  • Vitamin E
  • Aluminum Chloride
  • Selenium