Probing 3-hydroxyflavone for in vitro glycorandomization of flavonols by YjiC

Appl Environ Microbiol. 2013 Nov;79(21):6833-8. doi: 10.1128/AEM.02057-13. Epub 2013 Aug 23.


The glycosylation of five different flavonols, fisetin, quercetin, myricetin, kaempferol, and 3-hydroxyflavone, was achieved by applying YjiC. 3-Hydroxyflavone was selected as a probe for in vitro glycorandomization of all flavonols using diverse nucleotide diphosphate-d/l-sugars. This study unlocked the possibilities of the glycodiversification of flavonols and the generation of novel compounds as future therapeutics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / enzymology
  • Chromatography, High Pressure Liquid
  • Drug Discovery / methods*
  • Escherichia coli
  • Flavonoids / chemistry
  • Flavonoids / metabolism*
  • Glycosylation
  • Glycosyltransferases / metabolism*
  • Metabolic Engineering / methods*
  • Molecular Structure
  • Nucleoside Diphosphate Sugars / metabolism


  • Flavonoids
  • Nucleoside Diphosphate Sugars
  • Glycosyltransferases
  • 3-hydroxyflavone