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, 10 (10), 989-91

DMSO Enhances Electrospray Response, Boosting Sensitivity of Proteomic Experiments

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DMSO Enhances Electrospray Response, Boosting Sensitivity of Proteomic Experiments

Hannes Hahne et al. Nat Methods.

Abstract

We report that low percentages of dimethylsulfoxide (DMSO) in liquid chromatography solvents lead to a strong enhancement of electrospray ionization of peptides, improving the sensitivity of protein identification in bottom-up proteomics by up to tenfold. The method can be easily implemented on any LC-MS/MS system without modification to hardware or software and at no additional cost.

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