Transgene-free genome editing in Caenorhabditis elegans using CRISPR-Cas

Genetics. 2013 Nov;195(3):1167-71. doi: 10.1534/genetics.113.155879. Epub 2013 Aug 26.

Abstract

CRISPR-Cas is an efficient method for genome editing in organisms from bacteria to human cells. We describe a transgene-free method for CRISPR-Cas-mediated cleavage in nematodes, enabling RNA-homology-targeted deletions that cause loss of gene function; analysis of whole-genome sequencing indicates that the nuclease activity is highly specific.

Keywords: CRISPR; Caenorhabditis elegans; genome resequencing; reverse genetics; targeted nuclease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Caenorhabditis elegans / genetics*
  • Genes, Helminth
  • Genetic Engineering
  • Genome, Helminth*
  • Humans
  • Mutation
  • RNA Editing / genetics*
  • RNA, Helminth / genetics

Substances

  • RNA, Helminth