Heritable custom genomic modifications in Caenorhabditis elegans via a CRISPR-Cas9 system

Genetics. 2013 Nov;195(3):1181-5. doi: 10.1534/genetics.113.156075. Epub 2013 Aug 26.


We adapted the CRISPR-Cas9 system for template-mediated repair of targeted double-strand breaks via homologous recombination in Caenorhabditis elegans, enabling customized and efficient genome editing. This system can be used to create specific insertions, deletions, and base pair changes in the germline of C. elegans.

Keywords: CRISPR–Cas9; Caenorhabditis elegans; genome editing; germline; homologous recombination.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Caenorhabditis elegans / genetics*
  • DNA Breaks, Double-Stranded
  • DNA, Helminth / genetics
  • Gene Targeting
  • Genetic Engineering
  • Genome, Helminth*
  • Homologous Recombination
  • Mutagenesis
  • Mutagenesis, Insertional
  • RNA Editing / genetics


  • DNA, Helminth