CRISPR/Cas9-targeted mutagenesis in Caenorhabditis elegans

Genetics. 2013 Nov;195(3):1187-91. doi: 10.1534/genetics.113.156299. Epub 2013 Aug 26.


The generation of genetic mutants in Caenorhabditis elegans has long relied on the selection of mutations in large-scale screens. Directed mutagenesis of specific loci in the genome would greatly speed up analysis of gene function. Here, we adapt the CRISPR/Cas9 system to generate mutations at specific sites in the C. elegans genome.

Keywords: CRISPR; Caenorhabditis elegans; Cas9; double-strand break; genome engineering.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • CRISPR-Cas Systems*
  • Caenorhabditis elegans / genetics*
  • DNA, Helminth / genetics
  • Gene Targeting
  • Genome, Helminth
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed*


  • DNA, Helminth