Production of enzymes by Alteromonas sp. A321 to degrade polysaccharides from Enteromorpha prolifera

Abstract

Polysaccharides from Enteromorpha prolifera (PE) are becoming increasingly popular due to its bioactivity and abundant source. Screening novel microorganisms which could secrete enzymes to degrade PE efficiently for oligosaccharides production is a promising solution to improve its application. In this study, a marine bacterium that can produce enzymes to degrade PE specifically was selected. It was identified as Alteromonas sp. A321, based on the biochemical properties and 16S rDNA gene sequencing. In order to maximize the activity of degradase for polysaccharides from E. prolifera (DPE), the effects of medium composition and culture conditions were investigated. The highest DPE production was obtained in the medium consisting of K2HPO4 0.15%, PE 0.9%, NaNO3 0.4%, NaCl 1.0% and MgSO4 0.05%. The degradase activity was enhanced from original 0.391 U/ml to 0.744 U/ml. DPE show high efficiency and substrate specificity to PE with 63.53% of reducing sugar production in the 7 h hydrolysis.

Keywords: Alteromonas sp. A321; DPE; Degradase; Enzymatic hydrolysis; Identification; PE; Polysaccharides from Enteromorpha prolifera; Response surface methodology; degrdase for polysaccharides from Enteromorpha prolifera; polysaccharides from Enteromorpha prolifera.