Molecular cloning and expression pattern of 11 genes involved in lipid metabolism in yellow catfish Pelteobagrus fulvidraco

Abstract

11 genes involved in lipid metabolism were cloned from liver of yellow catfish Pelteobagrus fulvidraco, including CPT 1A, CPT 1B, PPARα, PPARγ, SREBP-1, G6PD, 6PGD, FAS, acetyl-CoA ACCa, ACCb, and LPL. Phylogenetic analysis further identified these genes, and confirmed the classification and evolutionary status of yellow catfish. mRNA of all eleven genes was present in liver, muscle, mesenteric adipose, ovary and heart, but at varying levels. The present study will facilitate further studies on the regulation of lipid metabolism at the molecular level for the fish species.

Keywords: 6-phosphogluconate dehydrogenase; 6PGD; ACC; CPT I; EF1α; FAS; G6PD; GAPDH; Gene cloning; HPRT; LPL; Lipid metabolism; MS-222; NJ; ORF; PCR; PPAR; Pelteobagrus fulvidraco; SEM; SREBP; Tissue expression; acetyl-CoA carboxylase; carnitine palmitoyltransferase; elongation factor 1 alpha; fatty acid synthase; glucose 6-phosphate dehydrogenase; glyceraldehydes 3-phosphate dehydrogenase; hypoxanthine-guanine phosphoribosyl transferase; lipoprotein lipase; neighbor-joining; open reading frame; peroxisome proliferators-activated receptor; polymerase chain reaction; standard error of mean; sterol-regulator element-binding protein; tricaine methanesulfonate.