Abstract
The pea rbcS-3A promoter with a 5' deletion to -166 (-166 rbcS-3A) contains two GT-1-binding sites. Mutational analyses demonstrated that a decrease in affinity for GT-1 correlates with reduced promoter activity. Transcription of -166 rbcS-3A in transgenic etiolated seedlings is induced by red light and suppressed by far-red light, indicating that it contains a phytochrome-responsive element.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Binding Sites
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DNA, Recombinant
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Fabaceae / genetics*
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Genes, Plant
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Light
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Molecular Sequence Data
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Phytochrome / metabolism*
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Plant Proteins / metabolism*
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Plants, Medicinal*
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Promoter Regions, Genetic*
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Regulatory Sequences, Nucleic Acid*
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Restriction Mapping
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Transcription, Genetic*
Substances
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DNA, Recombinant
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Plant Proteins
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Phytochrome