Autoimmune gastro-pancreatitis with anti-protein disulfide isomerase-associated 2 autoantibody in Aire-deficient BALB/cAnN mice

PLoS One. 2013 Aug 26;8(8):e73862. doi: 10.1371/journal.pone.0073862. eCollection 2013.

Abstract

Although the autoimmune regulator (Aire) knockout (KO) mouse model has been reported to present various organ-specific autoimmune diseases depending on genetic background, autoimmune pancreatitis in mice of BALB/c background has not yet been reported. Here, we report that Aire KO mice with BALB/cAnN background showed significant lymphoid cell infiltration in the pancreas and stomach. To examine whether the phenotype in the pancreas and stomach is due to autoimmune reaction associated with autoantibody production, indirect immunofluorescence staining followed by Western blot analysis was performed. Consequently, the autoantibody against pancreas and stomach was detected in the sera of Aire KO mice, and the target antigen of the autoantibody was identified as protein disulfide isomerase-associated 2 (Pdia2), which was reported to be expressed preferentially in the pancreas and stomach. Thus, Aire KO mice of BALB/cAnN background can serve as a useful animal model for autoimmune gastro-pancreatitis with anti-Pdia2 autoantibody production.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Autoantibodies / immunology*
  • Base Sequence
  • Blotting, Western
  • DNA Primers
  • Gastritis / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Molecular Sequence Data
  • Pancreatitis / immunology*
  • Polyendocrinopathies, Autoimmune / immunology*
  • Protein Disulfide-Isomerases / immunology*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Autoantibodies
  • DNA Primers
  • Protein Disulfide-Isomerases

Grant support

This work was supported by JSPS KAKENHI Grant Number 21790423, 24790555 and URL: http://www.jsps.go.jp/english/index.html. The funders had no role in study designm data collection and analysis, decision to publish, or preparation of the manuscript.