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. 2013 Jun 5;8(2):264-78.
doi: 10.4056/sigs.3496989. eCollection 2013.

Non contiguous-finished genome sequence and description of Bacillus massiliosenegalensis sp. nov

Dhamodharan Ramasamy  1 , Jean-Christophe Lagier, Aurore Gorlas, Didier Raoult, Pierre-Edouard Fournier
Affiliations

Non contiguous-finished genome sequence and description of Bacillus massiliosenegalensis sp. nov

Dhamodharan Ramasamy et al. Stand Genomic Sci. .

Abstract

Bacillus massiliosenegalensis strain JC6(T) sp. nov. is the type strain of Bacillus massiliosenegalensis sp. nov., a new species within the genus Bacillus. This strain was isolated from the fecal flora of a healthy Senegalese patient. B. massiliosenegalensis is an aerobic Gram-positive rod-shaped bacterium. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,981,278-bp long genome comprises a 4,957,301-bp chromosome and a 23,977-bp plasmid. The chromosome contains 4,925 protein-coding and 72 RNA genes, including 4 rRNA genes. The plasmid contains 29 protein-coding genes.

Keywords: Bacillus massiliosenegalensis; culturomics; genome; taxono-genomics.

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Figures

Figure 1
Figure 1
Phylogenetic tree showing the position of Bacillus massiliosenegalensis strain JC6T relative to other type strains within the Bacillus genus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum-likelihood method within the MEGA software. Numbers at the nodes are percentages of bootstrap values obtained by repeating the analysis 500 times to generate a majority consensus tree. Clostridium butyricum was used as outgroup. The scale bar represents a 2% nucleotide sequence divergence.
Figure 2
Figure 2
Gram staining of B. massiliosenegalensis strain JC6T
Figure 3
Figure 3
Transmission electron microscopy of B. massiliosenegalensis strain JC6T, using a Morgani 268D (Philips) at an operating voltage of 60kV. The scale bar represents 500 nm.
Figure 4
Figure 4
Reference mass spectrum from B. massiliosenegalensis strain JC6T. Spectra from 12 individual colonies were compared and a reference spectrum was generated.
Figure 5
Figure 5
Gel view comparing B. massiliosenegalensis sp. nov strain JC6T and other Bacillus species. The gel view displays the raw spectra of loaded spectrum files arranged in a pseudo-gel like look. The x-axis records the m/z value. The left y-axis displays the running spectrum number originating from subsequent spectra loading. The peak intensity is expressed by a Gray scale scheme code. The color bar and the right y-axis indicate the relation between the color withwhich a peak is displayed and the peak intensity in arbitrary units. Displayed species are indicated on the left.
Figure 6
Figure 6
Graphical circular map of the chromosome. From outside to the center: Genes on the forward strand (colored by COG categories), genes on the reverse strand (colored by COG categories), RNA genes (tRNAs green, rRNAs red), GC content, and GC skew.
Figure 7
Figure 7
Distribution of functional classes of predicted genes on B. massiliosenegalensis (dark blue), B. cereus (green), B. licheniformis (red), B. thuringiensis (yellow) B. timonensis (brown) and B. subtilis (light blue) chromosomes according to the clusters of orthologous groups of proteins.
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