MicroRNA-16 inhibits bladder cancer proliferation by targeting Cyclin D1

Asian Pac J Cancer Prev. 2013;14(7):4127-30. doi: 10.7314/apjcp.2013.14.7.4127.


MicroRNA-16 (miR-16) has been demonstrated to regulate proliferation and apoptosis in many types of cancers, but its biological function in bladder cancer remains unknown. Here, we found expression of miR-16 to be downregulated in bladder cancer in comparison with the adjacent normal tissues. Enforced expression of miR- 16 was able to inhibit cell proliferation in TCHu-1 cells, in line with results for miR-16 antisense oligonucleotides (antisense miR-16). At the molecular level, our results further revealed that cyclin D1 expression was negatively regulated by miR-16. Therefore, the data reported here demonstrate that miR-16 is an important regulator in bladder cancer, which will contribute to better understanding of important mis-regulated miRNAs.

MeSH terms

  • Apoptosis
  • Blotting, Western
  • Cell Cycle
  • Cell Proliferation*
  • Cyclin D1 / antagonists & inhibitors
  • Cyclin D1 / genetics*
  • Cyclin D1 / metabolism
  • Flow Cytometry
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Luciferases / metabolism
  • MicroRNAs / genetics*
  • Oligonucleotides, Antisense / pharmacology
  • RNA, Messenger / genetics
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Urinary Bladder Neoplasms / genetics
  • Urinary Bladder Neoplasms / metabolism
  • Urinary Bladder Neoplasms / pathology*


  • CCND1 protein, human
  • MIRN16 microRNA, human
  • MicroRNAs
  • Oligonucleotides, Antisense
  • RNA, Messenger
  • Cyclin D1
  • Luciferases