The effect of processing on chlorogenic acid content of commercially available coffee

Food Chem. 2013 Dec 15;141(4):3335-40. doi: 10.1016/j.foodchem.2013.06.014. Epub 2013 Jun 13.


Chlorogenic acids (CGA) are a class of polyphenols noted for their health benefits. These compounds were identified and quantified, using LC-MS and HPLC, in commercially available coffees which varied in processing conditions. Analysis of ground and instant coffees indicated the presence of caffeoylquinic acids (CQA), feruloylquinic acids (FQA) and dicaffeoylquinic acids (diCQA) in all 18 samples tested. 5-CQA was present at the highest levels, between 25 and 30% of total CGA; subsequent relative quantities were: 4-CQA>3-CQA>5-FQA>4-FQA>diCQA (sum of 3,4, 3,5 and 4,5-diCQA). CGA content varied greatly (27.33-121.25mg/200 ml coffee brew), driven primarily by the degree of coffee bean roasting (a high amount of roasting had a detrimental effect on CGA content). These results highlight the broad range of CGA quantity in commercial coffee and demonstrate that coffee choice is important in delivering optimum CGA intake to consumers.

Keywords: Chlorogenic acids; Coffee (Coffea spp.); Decaffeinated coffee; Instant coffee; Roasting.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chlorogenic Acid / chemistry*
  • Chromatography, High Pressure Liquid
  • Coffea / chemistry*
  • Coffee / chemistry*
  • Cooking / methods*
  • Hot Temperature
  • Mass Spectrometry
  • Molecular Structure


  • Coffee
  • Chlorogenic Acid