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Comparative Study
, 141 (4), 3537-45

Comparison of Different Methods for Extraction From Tetraclinis Articulata: Yield, Chemical Composition and Antioxidant Activity

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Comparative Study

Comparison of Different Methods for Extraction From Tetraclinis Articulata: Yield, Chemical Composition and Antioxidant Activity

Nejia Herzi et al. Food Chem.

Abstract

In the present study, three techniques of extraction: hydrodistillation (HD), solvent extraction (conventional 'Soxhlet' technique) and an innovative technique, i.e., the supercritical fluid extraction (SFE), were applied to ground Tetraclinis articulata leaves and compared for extraction duration, extraction yield, and chemical composition of the extracts as well as their antioxidant activities. The extracts were analyzed by GC-FID and GC-MS. The antioxidant activity was measured using two methods: ABTS(•+) and DPPH(•). The yield obtained using HD, SFE, hexane and ethanol Soxhlet extractions were found to be 0.6, 1.6, 40.4 and 21.2-27.4 g/kg respectively. An original result of this study is that the best antioxidant activity was obtained with an SFE extract (41 mg/L). The SFE method offers some noteworthy advantages over traditional alternatives, such as shorter extraction times, low environmental impact, and a clean, non-thermally-degraded final product. Also, a good correlation between the phenolic contents and the antioxidant activity was observed with extracts obtained by SFE at 9 MPa.

Keywords: Antioxidant activity; Hydrodistillation; Soxhlet extraction; Supercritical carbon dioxide; Tetraclinis articulata essential oil.

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