Isolation and identification of chitin in three-dimensional skeleton of Aplysina fistularis marine sponge

Int J Biol Macromol. 2013 Nov;62:94-100. doi: 10.1016/j.ijbiomac.2013.08.039. Epub 2013 Aug 28.

Abstract

The recent discovery of chitin within skeletons of numerous marine and freshwater sponges (Porifera) stimulates further experiments to identify this structural aminopolysaccharide in new species of these aquatical animals. Aplysina fistularis (Verongida: Demospongiae: Porifera) is well known to produce biologically active bromotyrosines. Here, we present a detailed study of the structural and physico-chemical properties of the three-dimensional skeletal scaffolds of this sponge. Calcofluor white staining, Raman and IR spectroscopy, ESI-MS as well as chitinase digestion test were applied in order to unequivocally prove the first discovery of α-chitin in skeleton of A. fistularis.

Keywords: Chitin; Marine sponges; Scaffold.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aquatic Organisms / chemistry*
  • Chitin / analysis*
  • Chitin / chemistry
  • Chitin / isolation & purification*
  • Porifera / anatomy & histology*
  • Porifera / chemistry*
  • Skeleton*

Substances

  • Chitin