Quantitative Analysis of Cellular Lipids by Nano-Electrospray Ionization Mass Spectrometry

Methods Mol Biol. 2013;1033:3-20. doi: 10.1007/978-1-62703-487-6_1.

Abstract

Lipid analysis performed by nano-electrospray ionization mass spectrometry is a highly sensitive method for quantification of lipids including all lipid species of a given lipid class. Various instrumental setups are used for quantitative lipid analysis, including different modes of ionization, separation, and detection. Here we describe a work-flow for the rapid and quantitative analysis of lipid species from cellular membranes by direct infusion of lipid extracts to a nano-electrospray ionization triple quadrupole/linear ion trap mass spectrometer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chemical Fractionation / methods
  • Lipids / analysis
  • Lipids / chemistry*
  • Mass Spectrometry / instrumentation
  • Mass Spectrometry / methods*
  • Reference Standards

Substances

  • Lipids