A novel partner of Scalloped regulates Hippo signaling via antagonizing Scalloped-Yorkie activity

Cell Res. 2013 Oct;23(10):1201-14. doi: 10.1038/cr.2013.120. Epub 2013 Sep 3.

Abstract

The Hippo (Hpo) pathway controls tissue growth and organ size by regulating the activity of transcriptional co-activator Yorkie (Yki), which associates with transcription factor Scalloped (Sd) in the nucleus to promote downstream target gene expression. Here we identify a novel protein Sd-Binding-Protein (SdBP)/Tgi, which directly competes with Yki for binding to Sd through its TDU domains and inhibits the Sd-Yki transcriptional activity. We also find that SdBP retains Yki in the nucleus through the association with Yki WW domains via its PPXY motifs. Collectively, we identify SdBP as a novel component of the Hpo pathway, negatively regulating the transcriptional activity of Sd-Yki to restrict tissue growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs
  • Animals
  • Carrier Proteins / analysis
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Drosophila / genetics
  • Drosophila / growth & development*
  • Drosophila / metabolism
  • Drosophila Proteins / analysis
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Gene Expression Regulation, Developmental
  • Intracellular Signaling Peptides and Proteins / metabolism*
  • Nuclear Proteins / analysis
  • Nuclear Proteins / metabolism*
  • Protein Interaction Maps
  • Protein Structure, Tertiary
  • Protein-Serine-Threonine Kinases / metabolism*
  • Signal Transduction
  • Trans-Activators / analysis
  • Trans-Activators / metabolism*
  • Transcription Factors / analysis
  • Transcription Factors / metabolism*
  • Transcriptional Activation
  • Up-Regulation

Substances

  • Carrier Proteins
  • Drosophila Proteins
  • Intracellular Signaling Peptides and Proteins
  • Nuclear Proteins
  • Tgi protein, Drosophila
  • Trans-Activators
  • Transcription Factors
  • Yki protein, Drosophila
  • sd protein, Drosophila
  • Protein-Serine-Threonine Kinases
  • hpo protein, Drosophila