Mouse and human islets survive and function after coating by biosilicification

Am J Physiol Endocrinol Metab. 2013 Nov 15;305(10):E1230-40. doi: 10.1152/ajpendo.00081.2013. Epub 2013 Sep 3.


Inorganic materials have properties that can be advantageous in bioencapsulation for cell transplantation. Our aim was to engineer a hybrid inorganic/soft tissue construct by inducing pancreatic islets to grow an inorganic shell. We created pancreatic islets surrounded by porous silica, which has potential application in the immunoprotection of islets in transplantation therapies for type 1 diabetes. The new method takes advantage of the islet capsule surface as a template for silica formation. Mouse and human islets were exposed to medium containing saturating silicic acid levels for 9-15 min. The resulting tissue constructs were then cultured for up to 4 wk under normal conditions. Scanning electron microscopy and energy dispersive X-ray spectroscopy was used to monitor the morphology and elemental composition of the material at the islet surface. A cytokine assay was used to assess biocompatibility with macrophages. Islet survival and function were assessed by confocal microscopy, glucose-stimulated insulin release assays, oxygen flux at the islet surface, expression of key genes by RT-PCR, and syngeneic transplant into diabetic mice.

Keywords: coating; encapsulation; islet; silica; tissue engineering.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cell Survival / physiology
  • Coated Materials, Biocompatible / chemistry
  • Diabetes Mellitus, Type 1 / therapy
  • Drug Compounding / methods*
  • Humans
  • Islets of Langerhans / cytology*
  • Islets of Langerhans / physiology*
  • Islets of Langerhans Transplantation / methods
  • Mice
  • Oxygen / metabolism
  • Phase Transition
  • Silicon Dioxide / chemistry*
  • Tissue Engineering / methods


  • Coated Materials, Biocompatible
  • Silicon Dioxide
  • Oxygen