Low titer lentiviral transgenesis in rodents with simian immundeficiency virus vector

Biotechniques. 2013 Sep;55(3):137-40. doi: 10.2144/000114078.


Efficient production of transgenic animals using low-titer lentiviral constructs remains challenging. Here we demonstrate that microinjection of simian immundeficiency virus-derived lentiviral constructs can produce transgenic mice and rats with high efficiency even when using low-titer virus preparations.

Keywords: Lentiviral transgenesis; SIV; low titer; transgenic mice; transgenic rat.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified / genetics*
  • Embryo, Mammalian
  • Female
  • Gene Transfer Techniques*
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Jurkat Cells
  • Mice
  • Micromanipulation
  • Rats
  • Rats, Wistar
  • Simian Immunodeficiency Virus / genetics*


  • enhanced green fluorescent protein
  • Green Fluorescent Proteins