Diamine oxidase as a marker of intestinal mucosal injury and the effect of soluble dietary fiber on gastrointestinal tract toxicity after intravenous 5-fluorouracil treatment in rats

Med Mol Morphol. 2014 Jun;47(2):100-7. doi: 10.1007/s00795-013-0055-7. Epub 2013 Sep 5.


The level of plasma diamine oxidase (DAO) activity is associated with the maturation and integrity of small intestinal mucosa. This study in rats investigated whether a decreased level of plasma DAO could reflect the severity of mucosal injury due to intravenous 5-fluorouracil (5-FU) treatment. The beneficial effect of soluble dietary fiber (SDF) on preventing diarrhea after 5-FU treatment was also examined. To induce diarrhea, 5-FU (50 mg/kg/day for four days) was administered via the tail vein with or without SDF supplementation. After 5-FU treatment, the majority of rats developed moderate to severe diarrhea, and levels of plasma DAO activity significantly decreased compared to those of control group (P < 0.05). Scanning electron microscopy revealed disarrangement of the small intestinal villi. Contrarily, the rats supplemented with SDF had diarrhea less frequently (50.0 vs. 91.7 %, P = 0.025) on day five, and DAO activity levels were significantly higher than in those rats administered 5-FU alone (8.25 ± 5.34 vs. 5.50 ± 4.32, P = 0.023). In conclusion, plasma DAO activity decreases in response to severe intestinal mucosal injury after 5-FU treatment, and SDF supplementation might be a practical and useful treatment for reducing the intestinal toxicity of 5-FU.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Intravenous
  • Amine Oxidase (Copper-Containing) / blood*
  • Animals
  • Biomarkers / blood*
  • Diarrhea / chemically induced
  • Diarrhea / prevention & control*
  • Dietary Fiber / pharmacology*
  • Fluorouracil / administration & dosage
  • Fluorouracil / toxicity*
  • Immunohistochemistry
  • Intestinal Mucosa / drug effects
  • Intestinal Mucosa / injuries*
  • Intestinal Mucosa / metabolism
  • Microscopy, Electron, Scanning
  • Rats
  • Statistics, Nonparametric


  • Biomarkers
  • Dietary Fiber
  • Amine Oxidase (Copper-Containing)
  • Fluorouracil