Localization of the mRNA for the 5-HT2 receptor by in situ hybridization histochemistry. Correlation with the distribution of receptor sites

Brain Res. 1990 Jul 30;524(1):139-43. doi: 10.1016/0006-8993(90)90502-3.


32P-labelled oligonucleotides complementary to rat 5-HT2 receptor mRNA were used as probes to study the distribution of cells in rat brain containing the mRNA coding for this receptor by in situ hybridization histochemistry. 5-HT2 receptor binding sites were visualized by autoradiography using [125I]DOI as ligand. Both distributions were comparable, demonstrating that 5-HT2 receptors are expressed by cells intrinsic to the neocortex (lamina Va), claustrum, olfactory bulb and several nuclei of the brainstem.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Autoradiography
  • Blotting, Northern
  • Brain / metabolism*
  • Iodine Radioisotopes
  • Male
  • Nucleic Acid Hybridization
  • Oligonucleotide Probes
  • RNA, Messenger / analysis*
  • RNA, Messenger / genetics
  • Rats
  • Rats, Inbred Strains
  • Receptors, Serotonin / analysis
  • Receptors, Serotonin / genetics*
  • Receptors, Serotonin / metabolism


  • Iodine Radioisotopes
  • Oligonucleotide Probes
  • RNA, Messenger
  • Receptors, Serotonin