Extracellular vesicle sizing and enumeration by nanoparticle tracking analysis

J Extracell Vesicles. 2013 Feb 15;2. doi: 10.3402/jev.v2i0.19671. eCollection 2013.


Nanoparticle tracking analysis (NTA) is a light-scattering technique that is useful for the rapid sizing and enumeration of extracellular vesicles (EVs). As a relatively new method, NTA has been criticised for a lack of standardisation. We propose the use of silica microspheres for the calibration of NTA measurements and describe in detail a protocol for the analysis of EVs by NTA which should minimise many of the sources of variability and imprecision associated with this technique.

Keywords: extracellular vesicles; light scattering; nanoparticle tracking analysis; standardisation.