How pure are your vesicles?

J Extracell Vesicles. 2013 Jan 10;2. doi: 10.3402/jev.v2i0.19861. eCollection 2013.


We propose a straightforward method to estimate the purity of vesicle preparations by comparing the ratio of nano-vesicle counts to protein concentration, using tools such as the increasingly available NanoSight platform and a colorimetric protein assay such as the BCA-assay. Such an approach is simple enough to apply to every vesicle preparation within a given laboratory, assisting researchers as a routine quality control step. Also, the approach may aid in comparing/standardising vesicle purity across diverse studies, and may be of particular importance in evaluating vesicular biomarkers. We herein propose some criteria to aid in the definition of pure vesicles.

Keywords: extracellular vesicles; nano-particle tracking; sample purity; vesicle counting.