Isolation and characterization of a novel cinosulfuron degrading Kurthia sp. from a methanogenic microbial consortium

Zhiyong Ruan; Shan Zhou; Shenghua Jiang; Lei Sun; Yi Zhai; Yanwei Wang; Chao Chen; Bin Zhao

doi:10.1016/j.biortech.2013.08.017

Isolation and characterization of a novel cinosulfuron degrading Kurthia sp. from a methanogenic microbial consortium

Bioresour Technol. 2013 Nov:147:477-483. doi: 10.1016/j.biortech.2013.08.017. Epub 2013 Aug 9.

Authors

Zhiyong Ruan¹, Shan Zhou², Shenghua Jiang³, Lei Sun⁴, Yi Zhai⁵, Yanwei Wang⁶, Chao Chen⁶, Bin Zhao⁷

Affiliations

¹ State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Microbial Resources Collection and Preservation, Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China.
² State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
³ Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305, USA.
⁴ Institute of Animal Husbandry, Heilongjiang Academy of Agricultural Sciences, Harbin 150000, China.
⁵ Institute of Agriculture Engineering, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China.
⁶ Key Laboratory of Microbial Resources Collection and Preservation, Institute of Agricultural Resources and Regional Planning, CAAS, Beijing 100081, China.
⁷ State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China. Electronic address: binzhao@mail.hzau.edu.cn.

PMID: 24012736
DOI: 10.1016/j.biortech.2013.08.017

Abstract

A novel bacterial strain LAM0713 was isolated from a methanogenic bacterial complexes and identified as Kurthia sp. based on morphological, cultural, physio-biochemical characteristics and analysis of 16S rDNA sequence. Strain LAM0713 was found to be capable of utilizing cinosulfuron as sole nitrogen source for growth over a wide range of temperature (20-40 °C) and pH (5.0-9.0). Response surface methodology was used to optimize the degradation conditions. Strain LAM0713 could efficiently degrade 92.4% of initially supplemented 50 mg·L(1) cinosulfuron under the optimum conditions (pH 6.9, 31.8 °C) within 5 days. Five intermediates formed during cinosulfuron degradation were detected by liquid chromatography mass spectrometry (LC-MS), and a metabolic pathway for cinosulfuron degradation was proposed via cleavage of the sulfonylurea bridge. It is the first report showing that Kurthia sp. strain could degrade sulfonylurea herbicides, suggesting that strain LAM0713 may provide new insight into microbial degradation of herbicides.

Keywords: Biodegradation; Cinosulfuron; Kurthia sp.; Novel species.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biodegradation, Environmental
Chromatography, High Pressure Liquid
Herbicides / metabolism*
Mass Spectrometry
Microbial Consortia*
Sulfonylurea Compounds / metabolism*

Substances

Herbicides
Sulfonylurea Compounds
cinosulfuron