Accurate measurement of nicotinamide adenine dinucleotide (NAD⁺) with high-performance liquid chromatography

Methods Mol Biol. 2013;1077:203-15. doi: 10.1007/978-1-62703-637-5_14.


Nicotinamide adenine dinucleotide (NAD(+)) plays a critical role in regulating numerous biological and physiological pathways including metabolism, inflammation, cancer, and aging in mammals. Here we describe a highly quantitative method with reverse-phase high-performance liquid chromatography (HPLC) for the determination of NAD(+) levels in cells and tissues. This methodology provides accurate, reliable, and reproducible results of NAD(+) measurement, which enables us to analyze various pathophysiological changes in NAD(+) levels in vitro and in vivo.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid / methods*
  • Chromatography, Reverse-Phase / methods*
  • Humans
  • Mice
  • NAD / analysis*
  • NAD / metabolism
  • NIH 3T3 Cells
  • Tandem Mass Spectrometry / methods*


  • NAD