IFN-γ-stimulated neutrophils suppress lymphocyte proliferation through expression of PD-L1

PLoS One. 2013 Aug 28;8(8):e72249. doi: 10.1371/journal.pone.0072249. eCollection 2013.

Abstract

During systemic inflammation different neutrophil subsets are mobilized to the peripheral blood. These neutrophil subsets can be distinguished from normal circulating neutrophils (CD16(bright)/CD62L(bright)), based on either an immature CD16(dim)/CD62L(bright) or a CD16(bright)/CD62L(dim) phenotype. Interestingly, the latter neutrophil subset is known to suppress lymphocyte proliferation ex vivo, but how neutrophils become suppressive is unknown. We performed transcriptome analysis on the different neutrophil subsets to identify changes in mRNA expression that are relevant for their functions. Neutrophil subsets were isolated by fluorescence-activated cell sorting from blood of healthy volunteers that were administered a single dose of lipopolysaccharide (2 ng/kg i.v.) and the transcriptome was determined by microarray analysis. Interestingly, the CD16(bright)/CD62L(dim) suppressive neutrophils showed an interferon-induced transcriptome profile. More importantly, IFN-γ, but not IFN-α or IFN-β stimulated neutrophils, acquired the capacity to suppress lymphocyte proliferation through the expression of programmed death ligand 1 (PD-L1). These data demonstrate that IFN-γ-induced expression of PD-L1 on neutrophils enables suppression of lymphocyte proliferation. Specific stimulation of neutrophils present at the inflammatory sites might therefore have a pivotal role in regulating lymphocyte-mediated inflammation and autoimmune disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B7-H1 Antigen / genetics
  • B7-H1 Antigen / metabolism*
  • Cell Communication
  • Cell Proliferation*
  • Cells, Cultured
  • Endotoxemia / immunology
  • Endotoxemia / metabolism
  • Gene Expression
  • Humans
  • Interferon-gamma / physiology*
  • Lipopolysaccharides / pharmacology
  • Male
  • Neutrophils / immunology
  • Neutrophils / metabolism*
  • T-Lymphocytes / physiology*
  • Transcriptome / immunology
  • Up-Regulation / immunology

Substances

  • B7-H1 Antigen
  • CD274 protein, human
  • Lipopolysaccharides
  • Interferon-gamma

Grant support

SdK is supported by ZonMw, the Netherlands Grant No. 85100003. JL was supported by the Nano Cluster of Technology Foundation (STW FES0901: FES HTSM). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.