Human (α2→6) and avian (α2→3) sialylated receptors of influenza A virus show distinct conformations and dynamics in solution

Biochemistry. 2013 Oct 15;52(41):7217-7230. doi: 10.1021/bi400677n. Epub 2013 Sep 27.


Differential interactions between influenza A virus protein hemagglutinin (HA) and α2→3 (avian) or α2→6 (human) sialylated glycan receptors play an important role in governing host specificity and adaptation of the virus. Previous analysis of HA-glycan interactions with trisaccharides showed that, in addition to the terminal sialic acid linkage, the conformation and topology of the glycans, while they are bound to HA, are key factors in regulating these interactions. Here, the solution conformation and dynamics of two representative avian and human glycan pentasaccharide receptors [LSTa, Neu5Ac-α(2→3)-Gal-β(1→3)-GlcNAc-β(1→3)-Gal-β(1→4)-Glc; LSTc, (Neu5Ac-α(2→6)-Gal-β(1→4)-GlcNAc-β(1→3)-Gal-β(1→4)-Glc] have been explored using nuclear magnetic resonance and molecular dynamics simulation. Analyses demonstrate that, in solution, human and avian receptors sample distinct conformations, topologies, and dynamics. These unique features of avian and human receptors in solution could represent distinct molecular characteristics for recognition by HA, thereby providing the HA-glycan interaction specificity in influenza.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Birds
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics
  • Hemagglutinin Glycoproteins, Influenza Virus / metabolism
  • Humans
  • Influenza A virus / genetics
  • Influenza A virus / metabolism*
  • Influenza in Birds / metabolism*
  • Influenza in Birds / virology
  • Influenza, Human / metabolism*
  • Influenza, Human / virology
  • Models, Molecular
  • N-Acetylneuraminic Acid / metabolism*
  • Polysaccharides / chemistry*
  • Polysaccharides / metabolism
  • Receptors, Virus / chemistry*
  • Receptors, Virus / metabolism


  • Hemagglutinin Glycoproteins, Influenza Virus
  • Polysaccharides
  • Receptors, Virus
  • N-Acetylneuraminic Acid