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. 2013;77(9):1860-6.
doi: 10.1271/bbb.130281. Epub 2013 Sep 7.

Construction of an Engineering Strain Producing High Yields of α-Transglucosidase via Agrobacterium Tumefaciens-Mediated Transformation of Asperillus Niger

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Construction of an Engineering Strain Producing High Yields of α-Transglucosidase via Agrobacterium Tumefaciens-Mediated Transformation of Asperillus Niger

Ming Li et al. Biosci Biotechnol Biochem. .
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Abstract

In this study, Agrobacterium tumefaciens-mediated transformation (ATMT) was used in breeding industrial strains for the purpose of improving α-transglucosidase production. Firstly, an efficient ATMT system for Asperillus niger was established by optimization of several influencing factors, in which transformation efficiency was improved up to 14-fold compared with the initial conditions. Furthermore, binary vector pBI-Glu containing an α-transglucosidase expression cassette was constructed and transferred into Agrobacterium tumefaciens LBA4404 in order to infect A. niger. By the efficient ATMT method, the gene for α-transglucosidase, driven by strong promoter PglaA (the glucoamylase gene promoter), had a high expression level in A. niger A-8 (25.02 U/mL). The optimized ATMT system was found to be effective and suitable for A. niger, and should be a useful tool for studying the function of A. niger genes and for industrial breeding of this strain.

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