Characterization of epiphytic bacterial communities from grapes, leaves, bark and soil of grapevine plants grown, and their relations

PLoS One. 2013 Aug 30;8(8):e73013. doi: 10.1371/journal.pone.0073013. eCollection 2013.

Abstract

Despite its importance in plant health and crop quality, the diversity of epiphytic bacteria on grape berries and other plant parts, like leaves and bark, remains poorly described, as does the role of telluric bacteria in plant colonization. In this study, we compare the bacterial community size and structure in vineyard soils, as well as on grapevine bark, leaves and berries. Analyses of culturable bacteria revealed differences in the size and structure of the populations in each ecosystem. The highest bacteria population counts and the greatest diversity of genera were found in soil samples, followed by bark, grapes and leaves. The identification of isolates revealed that some genera - Pseudomonas, Curtobacterium, and Bacillus - were present in all ecosystems, but in different amounts, while others were ecosystem-specific. About 50% of the genera were common to soil and bark, but absent from leaves and grapes. The opposite was also observed: grape and leaf samples presented 50% of genera in common that were absent from trunk and soil. The bacterial community structure analyzed by T-RFLP indicated similarities between the profiles of leaves and grapes, on the one hand, and bark and soil, on the other, reflecting the number of shared T-RFs. The results suggest an interaction between telluric bacterial communities and the epiphytic bacteria present on the different grapevine parts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics
  • Bacteria / growth & development*
  • Biodiversity
  • Colony Count, Microbial
  • France
  • Fruit / microbiology
  • Molecular Sequence Data
  • Phylogeny
  • Plant Bark / microbiology*
  • Plant Leaves / microbiology*
  • Polymorphism, Restriction Fragment Length
  • RNA, Ribosomal, 16S / genetics
  • Soil Microbiology*
  • Vitis / growth & development*
  • Vitis / microbiology*

Substances

  • RNA, Ribosomal, 16S

Associated data

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Grant support

This work was supported by the CIVB (Conseil Interprofessionnel du Vin de Bordeaux) and by Bordeaux Sciences Agro. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.