Characterization of a novel polyclonal anti-hypusine antibody

Springerplus. 2013 Aug 29;2:421. doi: 10.1186/2193-1801-2-421. eCollection 2013.


The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A.

Keywords: Antibody; Cell lines; Hypusine; Recombinant proteins; eIF5A.