Abstract
Electrochemiluminescence (ECL) on bipolar electrode (BPE) is a sensitive, portable, and low-cost approach which has been employed to detect DNA and proteins. Here, we develop an ultrasensitive method for intracellular mRNA assay based on mRNA-mediated reporter DNA liberation and Ru(bpy)3(2+)-conjugated silica nanoparticles (RuSi@Ru(bpy)3(2+)) tag-based signal amplification.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biosensing Techniques / instrumentation
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Biosensing Techniques / methods*
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Breast Neoplasms
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Electrochemical Techniques
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Electrodes
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Female
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Humans
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Luminescent Measurements
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MCF-7 Cells
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Proto-Oncogene Proteins c-myc / chemistry*
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Proto-Oncogene Proteins c-myc / genetics
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Proto-Oncogene Proteins c-myc / metabolism
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RNA, Messenger / chemistry*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Wireless Technology
Substances
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MYC protein, human
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Proto-Oncogene Proteins c-myc
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RNA, Messenger