Electrochemiluminescence detection of c-Myc mRNA in breast cancer cells on a wireless bipolar electrode

Methods Mol Biol. 2013:1039:169-79. doi: 10.1007/978-1-62703-535-4_15.

Abstract

Electrochemiluminescence (ECL) on bipolar electrode (BPE) is a sensitive, portable, and low-cost approach which has been employed to detect DNA and proteins. Here, we develop an ultrasensitive method for intracellular mRNA assay based on mRNA-mediated reporter DNA liberation and Ru(bpy)3(2+)-conjugated silica nanoparticles (RuSi@Ru(bpy)3(2+)) tag-based signal amplification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / instrumentation
  • Biosensing Techniques / methods*
  • Breast Neoplasms
  • Electrochemical Techniques
  • Electrodes
  • Female
  • Humans
  • Luminescent Measurements
  • MCF-7 Cells
  • Proto-Oncogene Proteins c-myc / chemistry*
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-myc / metabolism
  • RNA, Messenger / chemistry*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Wireless Technology

Substances

  • MYC protein, human
  • Proto-Oncogene Proteins c-myc
  • RNA, Messenger