High-throughput functional microRNA profiling using recombinant AAV-based microRNA sensor arrays

Methods Mol Biol. 2013:1039:261-77. doi: 10.1007/978-1-62703-535-4_21.

Abstract

There is a lack of methods for high-throughput functional microRNA (miRNA) profiling. In this chapter, we describe a recombinant adeno-associated virus-based miRNA sensor array (miRNA Asensor array), which is able to profile functional miRNAs in cultured cells. The preparation of an miRNA Asensor array and its usage are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Biosensing Techniques*
  • Cell Line
  • Cloning, Molecular
  • Cricetinae
  • Dependovirus / genetics*
  • Escherichia coli
  • Genetic Vectors
  • High-Throughput Screening Assays*
  • MicroRNAs / chemistry*
  • Oligonucleotide Array Sequence Analysis

Substances

  • MicroRNAs