Abstract
Neuronal regeneration occurs naturally in a few restricted mammalian brain regions, but its functional significance remains debated. Here we search for unique features in the synaptic outputs made by adult-born granule cell interneurons in the mouse olfactory bulb using optogenetic targeting of specific neuronal ages. We find that adult-born interneurons are resistant to presynaptic GABA(B)-mediated depression of GABA release compared with interneurons born just after birth that exhibit strong GABA(B) neuromodulation. Correlated with this functional change, we found altered localization of the GGABA(B)R1 protein within adult-born granule cells. These results suggest that adult neurogenesis produces a population of functionally unique GABAergic synapses in the olfactory bulb.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult Stem Cells / physiology*
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Age Factors
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Animals
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Animals, Newborn
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Bacterial Proteins / genetics
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Channelrhodopsins
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Female
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GABA Agents / pharmacology
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GABAergic Neurons / drug effects
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GABAergic Neurons / physiology*
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In Vitro Techniques
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Inhibitory Postsynaptic Potentials / drug effects
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Inhibitory Postsynaptic Potentials / genetics
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Luminescent Proteins / genetics
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Male
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Mice
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Mice, Inbred C57BL
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Neurogenesis / genetics
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Neurogenesis / physiology*
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Olfactory Bulb / cytology*
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Olfactory Bulb / growth & development
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Receptors, GABA-B / metabolism
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Sodium Channel Blockers / pharmacology
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Synapses / drug effects
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Synapses / genetics
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Synapses / physiology*
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Synapsins / genetics
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Tetrodotoxin / pharmacology
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Transduction, Genetic
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gamma-Aminobutyric Acid / metabolism
Substances
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Bacterial Proteins
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Channelrhodopsins
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GABA Agents
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Luminescent Proteins
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Receptors, GABA-B
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Sodium Channel Blockers
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Synapsins
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yellow fluorescent protein, Bacteria
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Tetrodotoxin
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gamma-Aminobutyric Acid