Oral contrast enhances the resolution of in-life NIS reporter gene imaging

Cancer Gene Ther. 2013 Nov;20(11):638-41. doi: 10.1038/cgt.2013.57. Epub 2013 Sep 13.

Abstract

Sodium iodide symporter (NIS) reporter gene imaging is an excellent technology for noninvasive cell fate determination in living animals unless the NIS-transduced cells reside in perigastric organs such as the spleen, liver, diaphragm, omentum, pancreas, perigastric lymph nodes or perigastric tumor deposits. Here we report that orally administered barium sulfate enhances CT definition of the stomach, masks background gamma ray emissions from the stomach and enhances signal detection from radiotracer uptake in NIS-transduced organs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Barium Sulfate* / administration & dosage
  • Cell Line, Tumor
  • Contrast Media* / administration & dosage
  • Female
  • Gene Transfer Techniques
  • Genes, Reporter / drug effects*
  • Heterografts
  • Humans
  • Iodine Radioisotopes* / administration & dosage
  • Mice
  • Mice, Nude
  • Multimodal Imaging / methods
  • Ovarian Neoplasms / diagnosis
  • Ovarian Neoplasms / diagnostic imaging
  • Ovarian Neoplasms / pathology
  • Stomach / anatomy & histology
  • Stomach / diagnostic imaging
  • Symporters / genetics*
  • Symporters / metabolism
  • Tomography, Emission-Computed, Single-Photon / methods
  • Tomography, X-Ray Computed / methods

Substances

  • Contrast Media
  • Iodine Radioisotopes
  • Symporters
  • Barium Sulfate
  • sodium-iodide symporter