Inhibitory potential of Grifola frondosa bioactive fractions on α-amylase and α-glucosidase for management of hyperglycemia

Biotechnol Appl Biochem. Jul-Aug 2013;60(4):446-52. doi: 10.1002/bab.1105.


This study examined the inhibitory effects of Grifola frondosa (GF), a medicinal mushroom popularly consumed in traditional medicine and health food, on digestive enzymes related to type 2 diabetes; chemical profiles and inhibitory kinetics of its bioactive fractions were also analyzed. Results showed that all GF extracts showed weak anti-α-amylase activity; however, strong anti-α-glucosidase activity was noted on GF n-hexane extract (GF-H). Further fractionation confirmed that compared with acarbose (a commercial α-glucosidase inhibitor), the nonpolar fraction of GF possessed a stronger anti-α-glucosidase activity but a weaker anti-α-amylase activity. These activities were not derived from ergosterol and ergosterol peroxide, two major compounds of this fraction. The inhibitory kinetics of GF-H on α-glucosidase was competitive inhibition. GF-H was as good as acarbose in inhibiting the starch digestion in vitro. Oleic acid and linoleic acid could be the major active constituents that have contributed to the potency of GF in inhibiting α-glucosidase activity.

Keywords: Grifola frondosa; bioactive components; medicinal mushroom; α-amylase; α-glucosidase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Digestion / drug effects
  • Enzyme Inhibitors / pharmacology*
  • Enzyme Inhibitors / therapeutic use
  • Glycoside Hydrolase Inhibitors*
  • Grifola / chemistry*
  • Hydrolysis / drug effects
  • Hyperglycemia / drug therapy*
  • Hyperglycemia / enzymology*
  • Kinetics
  • Starch / metabolism
  • alpha-Amylases / antagonists & inhibitors*
  • alpha-Amylases / metabolism
  • alpha-Glucosidases / metabolism


  • Enzyme Inhibitors
  • Glycoside Hydrolase Inhibitors
  • Starch
  • alpha-Amylases
  • alpha-Glucosidases