Polysome analysis of mammalian cells

Methods Enzymol. 2013;530:183-92. doi: 10.1016/B978-0-12-420037-1.00010-5.


To assess the global translational level of mammalian cells (see similar protocols for bacteria and yeast on Analysis of polysomes from bacteria, Polysome Profile Analysis - Yeast and Polysome analysis for determining mRNA and ribosome association in Saccharomyces cerevisiae).

Keywords: Cell lysate; Genome-wide analysis techniques; Immobilize ribosomes; Mammalian cells; Polysome analysis; Size fractionation; Sucrose density gradient centrifugation.

MeSH terms

  • Animals
  • Cell Fractionation / methods*
  • Centrifugation, Density Gradient / methods*
  • Genome
  • Humans
  • Polyribosomes / chemistry
  • Polyribosomes / genetics*
  • Protein Biosynthesis
  • RNA, Messenger / genetics
  • Ribosomes / genetics


  • RNA, Messenger