RNA purification--precipitation methods

Methods Enzymol. 2013;530:337-43. doi: 10.1016/B978-0-12-420037-1.00019-1.

Abstract

When working with RNA, the need often arises to concentrate a sample or purify it from various salts, nucleotides, and proteins. RNA precipitation is an easy and cost-effective method for the concentration of RNA, leaving a pellet that can be resuspended in the buffer of choice.

Keywords: Ammonium acetate; Ethanol precipitation; Lithium chloride precipitation; Precipitation methods; RNA purification; Sodium acetate.

MeSH terms

  • Acetates / chemistry
  • Animals
  • Chemical Precipitation*
  • Ethanol / chemistry
  • Lithium Chloride / chemistry
  • RNA / isolation & purification*
  • Sodium Acetate / chemistry

Substances

  • Acetates
  • Ethanol
  • Sodium Acetate
  • RNA
  • Lithium Chloride
  • ammonium acetate