Antibacterial property expressed by a novel calcium phosphate glass

J Biomed Mater Res B Appl Biomater. 2014 Apr;102(3):423-9. doi: 10.1002/jbm.b.33019. Epub 2013 Sep 11.


We have developed a calcium phosphate glass (CPG) doped with Zn(2+) or F(-) or combined Zn(2+) and F(-) ions, which are naturally found in the human body and play a dual role in bone formation and antibacterial activity. Previously, we have demonstrated that this family of CPGs has superior osteoconductive and resorbable properties in vivo. This study aimed to investigate the antibacterial property of CPGs incorporating Zn(2+) and/or F(-) . We used Streptococcus mutans as a model organism because it is one of the major human oral pathogens and an early colonizer, and it has been associated with several oral infections, such as dental caries, periodontitis, and peri-implantitis. CPGs of 0.01 and 0.05 g were incubated with S. mutans for 0, 2, 4, and 6 h. Serial dilutions were plated in triplicate and colony forming units were determined. The antimicrobial effect of CPG incorporating Zn(2+) or F(-) was greater than CPG incorporating both these ions. CPG without doping produced a moderate antimicrobial effect. This family of CPGs, previously shown to promote new bone formation in vivo, is demonstrated to have superior bactericidal properties.

Keywords: Streptococcus mutans; antimicrobial properties; calcium phosphate glass; fluoride; phosphate; zinc.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 3T3 Cells
  • Algorithms
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Biofilms / drug effects
  • Bone Development / drug effects
  • Calcium Phosphates / pharmacology*
  • Cell Adhesion
  • Glass*
  • Magnesium Oxide / chemistry
  • Mice
  • Microbial Sensitivity Tests
  • Molecular Weight
  • Osseointegration
  • Osteoclasts
  • Sodium Fluoride / chemistry
  • Spectrometry, Fluorescence
  • Streptococcus mutans / drug effects
  • Zinc Oxide / chemistry


  • Anti-Bacterial Agents
  • Calcium Phosphates
  • Magnesium Oxide
  • Sodium Fluoride
  • calcium phosphate
  • Zinc Oxide