Expression of transforming growth factor beta 2 during the differentiation of murine embryonal carcinoma and embryonic stem cells

Dev Biol. 1990 Jan;137(1):161-70. doi: 10.1016/0012-1606(90)90017-d.


Transforming growth factor beta 2 (TGF beta 2) mRNA expression was studied by Northern blot analysis in a range of feeder-independent murine embryonal carcinoma (EC) cells and in feeder-dependent EC and embryonic stem (ES) cells. TGF beta 2 transcripts were not detected in any undifferentiated cells including P19, F9, PC13, C1003, PSA-1, P10, and ES. Following induction of differentiation, however, TGF beta 2 became expressed, independently of the cell type formed. Retinoic acid (RA) addition and/or deprivation of the differentiation inhibiting activity of feeder cells resulted in the appearance of TGF beta 2 transcripts within 2 days. These kinetics correlated entirely with the first appearance of the protein; an anti-peptide antibody specifically recognizing TGF beta 2 did not stain P19 EC cells by immunofluorescence but 2-3 days after RA addition, a significant proportion of the population was strongly labeled. In addition, primitive endoderm cells emerging from the inner cell mass of substrate attached blastocysts stained brightly with anti-TGF beta 2, while the undifferentiated inner cell mass cells did not. Although all trophectoderm cells at the mid-blastocyst stage were stained, few had detectable levels of TGF beta 2 after plating on a substrate. Neither TGF beta 1 nor TGF beta 2 affected the growth of EC cells, but a range of differentiated derivatives were all inhibited, with TGF beta 2 being marginally more effective than TGF beta 1 at the same concentration.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Differentiation
  • Cell Line
  • Embryo, Mammalian / physiology*
  • Fluorescent Antibody Technique
  • Gene Expression*
  • Genes
  • Mice
  • RNA, Messenger / genetics*
  • Teratoma
  • Transcription, Genetic
  • Transforming Growth Factors / analysis
  • Transforming Growth Factors / biosynthesis
  • Transforming Growth Factors / genetics*
  • Tumor Cells, Cultured / cytology
  • Tumor Cells, Cultured / metabolism


  • RNA, Messenger
  • Transforming Growth Factors