Mutations in the bglY gene increase the frequency of spontaneous deletions in Escherichia coli K-12

Proc Natl Acad Sci U S A. 1990 Jan;87(1):360-3. doi: 10.1073/pnas.87.1.360.


A strong mutator effect has been observed in Escherichia coli K-12 strains mutated in the bglY gene (27 min). The frequency of point mutations is not modified in bglY mutant strains. In contrast, a strong increase in spontaneous generation of large deletions has been observed in these strains, both for chromosomal markers (10-fold increase of galETK-chlA deletions and 100-fold increase of ptsI-cysK deletions) and for plasmid DNA (100-fold increase of large deletions in the region located upstream of the chloramphenicol-resistance gene in plasmid pGR71). bglY mutations are recessive and can be complemented by a DNA fragment of 900 base pairs assumed to contain the entire bglY wild-type gene. This mutator effect is recA-independent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Deletion*
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics*
  • Genes, Bacterial*
  • Genotype
  • Mutation*
  • Plasmids
  • Restriction Mapping


  • DNA, Bacterial